Anti-HSD11B1 Antibody

HSD11B1 (hydroxysteroid 11-beta dehydrogenase 1) is a NADPH-dependent oxidoreductase that converts cortisone into active cortisol and shapes local glucocorticoid signaling in liver, adipose, and central nervous system tissue. The enzyme is widely studied in the context of obesity, type 2 diabetes, hepatic steatosis, and metabolic syndrome, where elevated HSD11B1 activity drives tissue-specific cortisol regeneration and contributes to insulin resistance.

This unconjugated rabbit polyclonal reagent from Antibodies.com is raised against HSD11B1 and validated for western blot and immunohistochemistry workflows. The antibody detects endogenous HSD11B1 in both human and mouse samples, supporting comparative studies that pair patient-derived biopsies with rodent disease models such as high-fat-diet-induced obesity.

The product ships as a 100 µl liquid aliquot. For western blot, whole-cell or tissue lysates prepared in RIPA buffer resolve the target near its predicted molecular weight. For immunohistochemistry, the antibody can be applied to formalin-fixed paraffin-embedded sections of liver and adipose tissue to localize HSD11B1 expression. The polyclonal format offers epitope redundancy, which is helpful when probing isoforms or truncated variants of the enzyme.

Store the vial refrigerated at 4°C for short-term use, or aliquot into single-use portions and freeze at -20°C for long-term storage; repeated freeze-thaw cycles should be avoided to preserve antibody activity. For research use only.