Anti-HRH2 Antibody

The histamine H2 receptor (HRH2) is a G protein-coupled receptor central to gastric acid regulation, smooth muscle relaxation, and several cardiac and immune signaling pathways, and the Antibodies.com Anti-HRH2 polyclonal antibody is a rabbit-derived reagent raised against this target. The antibody is supplied as 100 µl of unconjugated antiserum at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, and it has been validated for Western blot and immunofluorescence on human, mouse, and rat samples.

Receptor localization studies, histamine pharmacology research, and characterization of GPCR signaling in gastric and cardiac tissue are common applications. The polyclonal format offers multi-epitope recognition, which can be advantageous when probing receptors that show low abundance or conformational sensitivity in denaturing gels, and the cross-reactivity profile across three mammalian species supports comparative work between human biopsy material and rodent model systems.

For handling, store the vial at -20 degrees C in working aliquots to limit freeze-thaw cycles and protect from light; the 50% glycerol cryoprotectant keeps the antibody liquid at -20 degrees C without freezing. Sodium azide is included at 0.1% as a microbial preservative and should be considered when planning downstream live-cell or in vivo conjugation steps. Centrifuge the vial briefly before opening to recover the full 100 µl volume from the container wall.

Each lot is released with reactivity data confirming recognition of the HRH2 orthologs in human, mouse, and rat, supporting standard immunoblotting of cell and tissue lysates and indirect immunofluorescence on fixed sections in basic and translational research settings.