Anti-HPRT1 Antibody

HPRT1 (Hypoxanthine-Guanine Phosphoribosyltransferase) is a constitutively expressed housekeeping enzyme central to the purine salvage pathway, catalyzing the conversion of hypoxanthine and guanine to IMP and GMP. This rabbit-derived polyclonal reagent from Antibodies.com targets the endogenous human, mouse, and rat protein and is supplied as a 100 µl liquid aliquot for routine immunodetection workflows.

Researchers commonly apply the antibody to western blot and immunohistochemistry, where it functions both as a specific detection reagent for HPRT1 expression studies and as a stable loading-control reference alongside cytoskeletal or metabolic markers. The unconjugated format allows flexible pairing with HRP, fluorophore, or biotin secondary detection chemistries, and the polyclonal nature supports recognition of multiple epitopes for improved signal in formalin-fixed tissue sections and whole-cell lysates.

Loss-of-function mutations in HPRT1 are linked to Lesch-Nyhan syndrome and idiopathic gout, making this reagent relevant for studies of nucleotide metabolism, oxidative stress, and cell-cycle normalization across cancer and neuroscience models.