Anti-HPA1 Antibody

Antibodies.com offers this rabbit polyclonal reagent for laboratories working in transfusion medicine, alloimmunization research, and platelet biology. The antibody targets the human platelet alloantigen HPA1, also known as HPA-1a or PlA1, a polymorphic epitope located on the β3 integrin subunit (GPIIIa) that participates in platelet adhesion and aggregation. The HPA1 system features a single Leu/Pro dimorphism at residue 33 of β3, distinguishing the HPA-1a (Leu33) and HPA-1b (Pro33) alloforms.

Supplied as a 100 µl liquid aliquot from a rabbit host, the unconjugated antiserum is validated for Western blot (WB) and ELISA applications with human samples. Researchers use it to probe platelet lysates, characterize β3 integrin expression in engineered cell lines, and study the alloantibody responses that drive neonatal alloimmune thrombocytopenia (NAIT), post-transfusion purpura, and platelet transfusion refractoriness in pregnancy and transfusion settings.

For storage, keep the vial frozen at -20 °C in single-use aliquots to minimize repeated freeze-thaw cycles; for active bench use, store refrigerated at 4 °C with a protease inhibitor additive. The polyclonal format allows recognition of multiple epitopes across the HPA1/β3 region, supporting studies of both native and denatured protein preparations.

Because the reagent is unconjugated, it can be paired with HRP, biotin, fluorophore, or bead-coupled secondary antibodies to match specific detection chemistries in flow cytometry, immunohistochemistry, or immunoassay pipelines. This flexibility makes it a practical primary antibody for core facilities and translational research groups handling diverse HPA1-related projects.