Anti-HoxB4 Antibody

Produced by Antibodies.com, this rabbit polyclonal reagent targets human HoxB4, a homeodomain transcription factor implicated in hematopoietic stem cell maintenance, anterior-posterior axis specification during embryogenesis, and aberrant expression in certain leukemic contexts. The 100 µL unconjugated preparation is validated for Western blot and ELISA workflows, with confirmed reactivity against both human and mouse samples.

Raised against a region of the HoxB4 homeodomain, the polyclonal antiserum recognizes multiple epitopes on the native protein, supporting robust detection in transcription factor expression studies. Because HOXB4 localizes to the nucleus, nuclear extract preparations are recommended for downstream blotting to maximize signal. For long-term storage, keep aliquots frozen at -20 °C and limit freeze-thaw cycles; active working aliquots can be held briefly at 4 °C.

This reagent is a useful tool for developmental biology projects tracking HOXB4 during embryonic stem cell differentiation, for hematopoietic stem cell expansion assays in murine models, and for examining altered HOXB4 levels in leukemia cell line panels. It can also serve as a detection reagent in chromatin immunoprecipitation experiments when paired with appropriate isotype and pre-immune controls.