Anti-HNF4A (phospho S304) Antibody

This phospho-specific polyclonal antibody from Antibodies.com recognizes hepatocyte nuclear factor 4 alpha (HNF4A) only when serine 304 is phosphorylated, a post-translational modification linked to transcriptional control of hepatic gene programs governing gluconeogenesis, lipid transport, bile acid synthesis, and xenobiotic metabolism. Raised in rabbit and purified as an IgG preparation, the reagent is supplied unconjugated in a 50 µl vial, ready for direct incubation with transferred protein on nitrocellulose or PVDF membranes in standard western blot workflows. Researchers investigating hepatocellular differentiation, insulin signaling crosstalk, intestinal epithelial maturation, or colorectal and hepatic carcinoma biology rely on this reagent to monitor kinase-driven modulation of HNF4A activity in cell lysates and tissue homogenates, since phosphorylation at S304 has been associated with altered DNA-binding affinity, subcellular localization, and nuclear export dynamics. The antibody is validated for human reactivity and ships without conjugated fluorophores or enzymes, allowing laboratories to pair it with their own HRP- or alkaline phosphatase-labeled secondary reagents and chemiluminescent substrates.