Anti-HNF4A (phospho S304) Antibody

This phospho-specific polyclonal reagent from Antibodies.com recognizes human HNF4A only when the protein is phosphorylated at serine 304, enabling selective detection of the activated transcription factor in hepatocyte and pancreatic islet signaling studies. The antibody is produced in rabbit as an IgG fraction and provided unconjugated at 100 µl, ready for use in standard immunoblotting workflows after a brief blocking step with non-fat milk in TBST.

Validated for Western blot, the reagent detects phosphorylated HNF4A on nitrocellulose or PVDF membranes following SDS-PAGE separation of whole-cell lysates prepared in RIPA buffer supplemented with phosphatase inhibitors to preserve the labile S304 modification. Horseradish peroxidase or alkaline phosphatase-conjugated secondary antibodies are then applied, and the target band is visualized near the expected molecular weight of the transcription factor. The phospho-epitope specificity makes the reagent a useful tool for comparing basal versus stimulated HNF4A activation in hepatic differentiation models, diabetes research panels, and studies of nuclear receptor signaling.

For long-term stability, aliquot the antibody upon arrival and store at -20°C, avoiding repeated freeze-thaw cycles. Short-term working aliquots can be kept refrigerated for up to two weeks. Provided as a liquid formulation suitable for direct dilution into blocking buffer, the antibody is intended for research use only and is not validated for diagnostic or therapeutic applications.