Anti-HNF4α Antibody

HNF4α is a nuclear receptor transcription factor that governs hepatocyte identity, gluconeogenesis, lipid metabolism, and bile acid synthesis, making this rabbit polyclonal reagent a foundational tool for liver biology and metabolic disease research. Supplied by Antibodies.com as an unconjugated primary antibody in a 50 _l vial, the polyclonal antiserum is raised in rabbit and validated for Western blot, immunohistochemistry, and immunofluorescence workflows across human, mouse, and rat samples.

The antibody is formulated at 1 mg/ml in phosphate buffered saline (PBS) containing 0.05% sodium azide at approximately pH 7.2. For routine handling, store refrigerated at 4˚C and protect from light; the sodium azide preservative allows short-term use in standard immunoassays. Aliquoting upon first use helps minimize freeze-thaw cycles and preserves titre over the shelf life of the reagent.

HNF4α serves as a robust marker of differentiated hepatocytes and is widely used to study hepatocellular carcinoma progression, MODY1 diabetes mechanisms linked to HNF4α mutations, intestinal epithelial differentiation, and pancreatic beta-cell function. Because the target localizes to the nucleus, the antibody produces clean nuclear staining patterns in tissue sections and cultured cells, supporting morphologic and quantitative analyses in both fresh-frozen and formalin-fixed paraffin-embedded specimens. Researchers studying hepatic lineage commitment, drug-induced liver injury, and metabolic syndrome routinely deploy this reagent for cell culture characterization, chromatin studies, and validation of hepatic differentiation in stem-cell derived hepatocyte models.