Anti-HMGN5 Antibody

HMGN5 is a nucleosome-binding member of the high-mobility group N protein family that modulates chromatin architecture, transcription, and DNA repair, and aberrant HMGN5 expression has been documented in several human cancers and in pluripotent stem cell populations. This polyclonal reagent from Antibodies.com is raised in rabbit, isotype IgG, and supplied as 200 µl of unconjugated antiserum validated for Western blot detection of the human target. Comparable anti-HMGN5 antibodies have been used in published studies to probe overexpression in prostate, lung, gastric, and bladder carcinoma models, to track HMGN5 dynamics in embryonic and neural stem cell systems, and to confirm knockdown or knockout efficiency in CRISPR-edited lines. Standard Western blot workflows resolve whole-cell or tissue lysates by SDS-PAGE, transfer to PVDF or nitrocellulose membranes, and detect bound primary with anti-rabbit HRP or fluorescent secondary reagents, with primary dilution optimized empirically for each lysate. The polyclonal format broadens recognition across evolutionarily conserved epitopes of HMGN5, while the unconjugated presentation allows flexible pairing with user-preferred reporter chemistries and chromogens. Aliquot upon receipt and store refrigerated; protect from light and avoid repeated freeze-thaw cycles to preserve antibody activity.