Anti-HMGCR Antibody

Manufactured by Antibodies.com, this rabbit polyclonal reagent targets3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGCR), the rate-limiting enzyme of the mevalonate pathway, and is raised against a recombinant human HMGCR immunogen before being affinity-purified on the immunogen. Supplied as an unconjugated Ig preparation at 1.0 mg/mL in phosphate-buffered saline (pH 7.4) containing 150 mM NaCl, 0.02% sodium azide and 50% glycerol, the antibody is offered in a 50 µl format and is validated for Western blot and immunohistochemistry applications. The antibody specifically recognizes the endogenous total HMGCR protein in human, mouse and rat samples, making it suitable for comparative studies of cholesterol biosynthesis across species. Routine use involves the working dilutions recommended by Antibodies.com, with Western blot detection typically performed on extracts of cultured cell lines and IHC applied to fixed tissue sections. To preserve activity, the vial is shipped at 4 °C from the Antibodies.com distribution center in St. Louis, MO, and upon receipt should be aliquoted and stored at −20 °C, with repeated freeze/thaw cycles avoided. With a polyclonal clonality profile produced in rabbit, this reagent provides broad epitope coverage of HMGCR, supporting robust signal in both denaturing (WB) and fixed-tissue (IHC) workflows commonly used in cardiovascular, metabolic and oncology research laboratories.