Anti-HMGCL Antibody

Primary antibody from Antibodies.com targeting HMGCL, the mitochondrial enzyme 3-Hydroxy-3-Methylglutaryl-CoA Lyase that catalyzes the final step of hepatic ketogenesis and leucine catabolism. This rabbit polyclonal reagent is supplied as an unconjugated IgG preparation in a 200 µl liquid volume, validated for Western blot detection of the human HMGCL protein in cell and tissue lysates.

Researchers investigating HMG-CoA lyase deficiency, organic acidurias, mitochondrial fatty-acid oxidation disorders, and the regulation of ketone body production use this reagent to characterize endogenous expression across hepatocyte and fibroblast model systems. The polyclonal format allows recognition of multiple epitopes across the HMGCL polypeptide, which is helpful when working with denatured protein samples where individual linear determinants may be partially masked.

Typical workflow incorporates standard reducing SDS-PAGE, transfer to nitrocellulose or PVDF membrane, blocking, and primary incubation overnight at 4 °C, followed by an HRP- or fluorophore-conjugated secondary reagent for chemiluminescent readout on the blot.