Anti-HMG-1 Antibody

This rabbit polyclonal antibody targets High Mobility Group Box 1 (HMG-1/HMGB1), a highly conserved non-histone nuclear DNA-binding protein that plays dual roles in chromatin architecture and extracellular inflammatory signaling. Raised in rabbit and supplied as an unconjugated reagent, the antibody recognizes HMGB1 in human, mouse, and rat samples and is validated for Western blot and ELISA workflows. Each vial contains 100 µL of polyclonal antiserum suitable for detection of endogenous HMGB1 in cell lysates and tissue homogenates, supporting research into DAMP-mediated inflammation, sepsis pathology, tumor progression, and chromatin-associated transcription studies. Storage at -20 °C in working aliquots preserves reactivity across multiple freeze-thaw cycles, and the unconjugated format allows flexible pairing with HRP, fluorescent, or biotin secondary reagents depending on assay design. Researchers investigating HMGB1 release during immunogenic cell death, alarmin signaling, or nuclear translocation events can use this reagent from Antibodies.com as a primary detection tool in western blot and ELISA applications.