Anti-HLA-DRA Antibody

The HLA-DRA protein is the alpha subunit of the major histocompatibility complex class II HLA-DR heterodimer, a cell-surface glycoprotein essential for presenting extracellular peptide antigens to CD4+ T lymphocytes. Expression is concentrated on professional antigen-presenting cells such as B cells, dendritic cells, macrophages, and monocytes, and can be induced on activated T cells and certain epithelial or tumor cell populations under inflammatory conditions.

This rabbit polyclonal reagent from Antibodies.com is supplied as a 100 µl unconjugated volume and is validated for Western blot (WB) and immunohistochemistry (IHC). Cross-reactivity is reported against human, mouse, and rat samples, supporting translational work that spans clinical tissue specimens and corresponding rodent disease models. The polyclonal format recognizes multiple epitopes on the HLA-DRA target, which can help maintain signal across varying expression levels and reduce sensitivity to single-epitope masking.

For Western blot, investigators typically load whole-cell lysates from antigen-presenting cell lines or lymphoid tissue and resolve proteins by SDS-PAGE, looking for the mature alpha-chain band. In IHC, the antibody is used to map HLA-DRA distribution in formalin-fixed paraffin-embedded sections of spleen, lymph node, thymus, and tonsil, as well as in tumor specimens where MHC class II status is being assessed for immune infiltrate profiling.

Storage handling for affinity-purified polyclonal IgG reagents generally involves aliquoting upon arrival to limit freeze-thaw cycles, with long-term storage at -20°C and protection from light.