Anti-HLA-DPA1 Antibody

HLA-DPA1 encodes the alpha chain of the HLA-DP class II major histocompatibility complex heterodimer, which pairs with the HLA-DPB1-encoded beta chain on the surface of professional antigen-presenting cells. This unconjugated rabbit polyclonal IgG reagent from Antibodies.com is raised against the human target and validated for Western blot detection, with a working volume of 200 µl supplied in a single vial.

The antibody binds the alpha subunit, allowing researchers to monitor MHC class II expression levels and subunit composition in lymphoid tissues, B-cell and dendritic cell lines, and primary monocytes. HLA-DPA1 transcription is driven by the CIITA master regulator, and altered HLA-DP surface expression has been documented in autoimmune disease, viral immune evasion, and graft-versus-host responses following allogeneic transplantation, supporting studies in transplantation immunology, antigen presentation, and disease association.

As a polyclonal reagent, it recognizes multiple epitopes across the HLA-DPA1 protein, increasing the likelihood of detection in varied sample preparations and standard denaturing SDS-PAGE workflows. Recommended handling is storage at -20 °C in working aliquots to limit freeze-thaw cycles and preserve antibody activity across multiple experiments.