Anti-HK2 Antibody

This polyclonal reagent from Antibodies.com recognizes Hexokinase 2 (HK2), a mitochondrial-associated glycolytic enzyme that catalyzes the first committed step of glucose metabolism by phosphorylating glucose to glucose-6-phosphate. Raised in rabbit and supplied unconjugated in a 100 µl volume, the antibody has been validated for Western blot, immunohistochemistry, and immunofluorescence applications on human, mouse, and rat specimens.

HK2 is a focal point in cancer metabolism research, where many tumors upregulate the enzyme to sustain the Warburg effect, diverting glucose carbons toward lactate production even under oxygen-replete conditions. The reagent supports studies of metabolic reprogramming in glioblastoma and other malignancies, as well as baseline mapping in skeletal muscle, cardiac tissue, brain, and pancreatic islets, where HK2 expression shifts during development and in response to insulin signaling.

Typical workflows include probing cultured cell lysates and tissue homogenates by Western blot, localizing HK2 in fixed paraffin-embedded tumor sections, and visualizing mitochondrial association by immunofluorescence in adherent cell lines. The polyclonal format recognizes multiple epitopes across the HK2 sequence, aiding detection of truncated isoforms or post-translationally modified protein. Store refrigerated and protect from light; aliquot upon arrival to limit freeze-thaw cycles. For research use only.