Anti-Histone H4 (tri methyl K20) Antibody

A rabbit polyclonal IgG antibody from Antibodies.com, raised against Histone H4 with a tri-methyl modification at Lys20 (K20). The unconjugated antibody reacts with Human, Mouse and Rat samples and is supplied as a liquid in Phosphate Buffered Saline (pH 7.3) containing 50% glycerol and 0.02% sodium azide. The target protein has a molecular weight of 11 kDa, and the antibody is purified by affinity chromatography.

Applications listed by the supplier include Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, ChIP and ChIPseq; the manufacturer further lists WB, IHC, ICC/IF and Dot Blot among the validated uses. Recommended dilutions are Dot Blot 1:500–1:2,000, WB 1:500–1:2,000, IHC 1:50–1:200 and ICC/IF 1:50–1:200. The immunogen is a synthetic trimethylated peptide around K20 of human histone H4 (NP_003529.1), with the immunogen sequence HRKVL.

The product is shipped at 4°C; upon delivery it should be aliquoted and stored at −20°C, with freeze/thaw cycles avoided.