Anti-Histone H4 (sDi-Methyl R3) Antibody

An unconjugated rabbit polyclonal reagent from Antibodies.com detects histone H4 di-methylated at arginine 3, an epigenetic modification on the H4 N-terminal tail linked to chromatin state and transcriptional output. The reagent is raised against the dimethyl-R3 epitope and supplied as 100 µl at 1 mg/ml in PBS buffer containing 0.1% sodium azide and 50% glycerol, with reactivity confirmed against human, mouse, and rat samples.

Validated for Western blot, immunohistochemistry, and immunofluorescence, the antibody supports immunoblotting of acid-extracted histones or nuclear lysates, immunostaining of fixed cells and tissue sections, and chromatin imaging workflows. The polyclonal format provides recognition of multiple epitopes around the modified arginine residue, useful when surveying global H4R3 methylation levels across cell-based experiments and tissue samples. The glycerol-containing formulation supports storage at -20 °C, limiting freeze-thaw stress on aliquoted antibody stocks in routine lab inventories, and is supplied in a small vial sized for typical antibody panels.