Anti-Histone H4 (S1) Antibody

This rabbit polyclonal reagent from Antibodies.com targets histone H4 phosphorylated at serine 1, a modification at the extreme N-terminus of the H4 tail that has been reported in chromatin remodeling studies, including work on nucleosome dynamics during the DNA damage response. The antibody is supplied as 100 µL of liquid at 1 mg/ml in phosphate buffered saline (PBS) containing 0.05% sodium azide at approximately pH 7.2, and is unconjugated so that the researcher's preferred secondary reagent or covalent label can be applied.

Validated applications include Western blot, immunohistochemistry, and immunofluorescence, and reactivity has been confirmed against human, mouse, and rat samples. Phosphorylation at H4 serine 1 can be tracked in chromatin fractions, acid-extracted histones, and paraformaldehyde-fixed tissue sections, allowing comparison of modification state across treatment conditions or developmental stages.

For routine storage, keep the vial at -20°C in single-use aliquots to limit freeze-thaw cycles; once thawed, an aliquot remains usable for several weeks if kept refrigerated and handled aseptically. Because sodium azide interferes with certain conjugation chemistries and with live-cell applications, aliquots can be dialyzed or gel-filtered before covalent labeling. The reagent is a polyclonal antibody raised in rabbit, so each lot is delivered as a heterogeneous affinity-purified fraction rather than as a defined monoclonal clone, and researchers studying the histone code, chromatin biology, or epigenetic inheritance can pair it with chromatin immunoprecipitation, immunoblotting, and immunostaining workflows.