Anti-Histone H4 (Mono-Methyl K20) Antibody

Raised in rabbit and affinity-purified by epitope-specific chromatography to greater than 95% purity by SDS-PAGE, this polyclonal antibody from Antibodies.com is designed to detect endogenous histone H4 only when mono-methylated at lysine 20 (K20me1), making it a precise tool for studying this specific epigenetic mark. It is supplied at 50 µl in PBS containing 0.1% sodium azide and 50% glycerol as a 1 mg/ml stock, and is unconjugated for maximum flexibility in downstream labelling. The immunogen is a synthetic peptide conjugated to KLH corresponding to residues 1-100 of human histone H4 bearing the K20 mono-methyl modification, and the target protein runs at approximately 11 kDa, consistent with the small size of the core histone. Validated applications span Western blot, immunohistochemistry, immunofluorescence, immunoprecipitation, ChIP, and ChIP-seq, and the reagent has been confirmed to react with human, mouse, and rat samples. Shipped from Cambridge, UK, this research-grade reagent is intended for research use only and is not for diagnostic or therapeutic applications.