Anti-Histone H4 (Mono-Methyl K20) Antibody

This polyclonal antibody from Antibodies.com specifically targets histone H4 mono-methylated at lysine 20 (H4K20me1), an epigenetic modification associated with chromatin compaction, DNA damage response, and transcriptional regulation. Raised in rabbit and supplied unconjugated, the reagent is formulated at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol for long-term stability. Each vial contains 100 µl, sufficient for multiple downstream experiments.

The antibody is validated for Western blot, immunohistochemistry, immunofluorescence, immunoprecipitation, chromatin immunoprecipitation (ChIP), and ChIP-seq workflows. It shows reactivity with human, mouse, and rat samples, supporting comparative epigenetics studies across mammalian models. For storage, keep frozen at -20 °C in single-use aliquots and minimize freeze-thaw cycles to preserve binding activity. The polyclonal format recognizes multiple epitopes on H4K20me1, frequently yielding stronger enrichment signal in ChIP applications than narrower monoclonal alternatives.