Anti-Histone H4 (di methyl K20) Antibody

A rabbit polyclonal reagent from Antibodies.com targeting Histone H4 dimethylated at Lys20 (K20). It has been validated for Western blot (WB), immunohistochemistry (IHC), immunocytochemistry/immunofluorescence (ICC/IF), immunoprecipitation (IP), and chromatin immunoprecipitation (ChIP), and is additionally listed for ChIP-seq, with reactivity confirmed against Human, Mouse, and Rat samples. The unconjugated IgG antibody was raised against a synthetic dimethylated peptide around K20 of human histone H4 (NP_003529.1) and purified by affinity chromatography.

Supplied as a liquid in Phosphate Buffered Saline pH 7.3 containing 50% glycerol and 0.01% Thiomersal, with lot-specific concentration. The target protein has a molecular weight of 11 kDa. Recommended starting dilutions are WB 1:500–1:1,000, IHC 1:50–1:200, ICC/IF 1:50–1:200, IP 1:50–1:200, ChIP 1:20–1:100, and ChIP-seq 1:20–1:100. The product is shipped at 4°C; on receipt, aliquot and store at -20°C, avoiding repeated freeze/thaw cycles.