Anti-Histone H4 (asymmetric di methyl R3) Antibody

From Antibodies.com, this rabbit polyclonal antibody recognises Histone H4 when asymmetrically di-methylated at arginine 3 (R3). It is supplied unconjugated, is of IgG isotype, and reacts with human, mouse and rat samples. The catalogue listing indicates applications of WB, IHC, IF and IP.

The manufacturer's datasheet specifies that the antibody was raised against a synthetic asymmetric dimethylated peptide around R3 of human histone H4 (NP_003539.1), with an immunogen sequence beginning SGRGK. The corresponding protein has a molecular weight of 11 kDa. The reagent is provided as a liquid in phosphate buffered saline at pH 7.3, containing 50% glycerol and 0.02% sodium azide, and is purified by affinity chromatography. Validated applications listed by the manufacturer are WB, ICC/IF and IP, with recommended dilutions of WB 1:500-1:2,000, ICC/IF 1:50-1:200 and IP 1:50-1:100.

Storage guidance is to ship at 4°C, then upon receipt aliquot and store at -20°C, avoiding repeated freeze/thaw cycles.