Anti-Histone H4 Antibody

A rabbit polyclonal antibody supplied by Antibodies.com as a 100 µl unconjugated reagent for detection of histone H4 across human, mouse, and rat samples. Histone H4 is one of the four core histones that package genomic DNA into nucleosomes, the fundamental repeating units of chromatin, and is a frequent target in research on gene regulation, epigenetic marking, and chromatin remodeling.

The reagent is validated for western blot and ELISA workflows. Its polyclonal profile is advantageous for probing the H4 tail, where multiple post-translational modifications including acetylation and methylation act as markers of active or repressed chromatin states. In western blot, the antibody typically resolves an approximately 11 kDa band corresponding to the core histone, enabling normalization against total H4 in nuclear extract preparations.

Supplied unconjugated in a liquid vial format, the antibody can be paired with secondary antibodies of choice for chromogenic, fluorescent, or chemiluminescent readout. Researchers commonly apply it to investigate histone modification dynamics, transcription factor occupancy studies, and chromatin immunoprecipitation follow-up experiments. Storage and buffer composition details are provided on the accompanying product datasheet.