Anti-Histone H4 Antibody

Manufactured by Antibodies.com, this rabbit polyclonal primary antibody targets Histone H4 and is supplied as a 50 µl liquid formulation at 1 mg/mL in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. The immunogen is a synthesized peptide derived from human Histone H4 around the non-acetylation site of K12, and the antibody was affinity-purified from rabbit antiserum by affinity-chromatography using the epitope-specific immunogen, ensuring recognition of endogenous levels of Histone H4 protein. Validated applications include Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry/Immunofluorescence (ICC/IF) and ELISA, making it a versatile reagent for chromatin and nuclear marker studies. The unconjugated polyclonal antibody is raised in rabbit and demonstrates reactivity with Human, Mouse, Rat and Monkey samples, and is frequently used as a general nuclear loading control alongside related Histone H4 synonyms H4/A, H4C1, H4FA and HIST1H4A. The product is shipped at 4°C; upon delivery it should be aliquoted and stored at -20°C, with repeated freeze/thaw cycles avoided to preserve activity. For research use only, this Antibodies.com reagent provides reliable detection of Histone H4 across multiple standard immunoassay platforms.