Anti-Histone H4 Antibody

Histone H4 sits at the core of the nucleosome, and this rabbit polyclonal reagent from Antibodies.com is raised to detect it across chromatin-related workflows. The unconjugated antibody is supplied as a 100 µl vial and has been validated for western blot, immunohistochemistry, immunofluorescence, and ELISA applications.

Cross-species reactivity covers human, mouse, rat, and monkey samples, making it useful as a chromatin loading control or for total histone H4 detection across model organisms and primary cell lines. The unconjugated format leaves the choice of secondary conjugate to the researcher, so HRP, biotin, Alexa Fluor, or alkaline phosphatase detection can be paired to fit the assay platform.

For working stocks, keep refrigerated between uses; aliquot to limit freeze-thaw cycles and protect from light when paired with a fluorophore. The polyclonal preparation ensures recognition of multiple epitopes along the histone H4 sequence, which is helpful when probing either unmodified or post-translationally modified variants in cell lysates and tissue sections.