Anti-Histone H4 Antibody

Histone H4 sits at the core of the eukaryotic nucleosome, where pairs of H4 proteins join H2A, H2B, and H3 to form the histone octamer that packages nuclear DNA into chromatin. This rabbit polyclonal IgG reagent from Antibodies.com was raised against the conserved Histone H4 protein and is supplied as 50 µL of unconjugated antiserum, leaving laboratories free to conjugate it directly to biotin, HRP, or a fluorophore of choice.

The polyclonal format recognizes multiple epitopes on denatured or fixed target, which is helpful for Western blot (WB) and immunofluorescence (IF) workflows, the two applications validated for this antibody. The reagent cross-reacts with Human, Mouse, and Rat samples, allowing the same vial to support experiments across common mammalian cell lines, primary tissue preparations, and standard model organisms. Histone H4 carries a dense set of post-translational marks — acetylation, methylation, phosphorylation, and ubiquitination — that are widely mapped in chromatin and epigenetics research, including work on nucleosome assembly, chromatin remodeling, and the DNA damage response.

For best results, aliquot upon receipt to limit repeated freeze-thaw cycles and store refrigerated to preserve antibody activity across the working shelf life.