Anti-Histone H4 Antibody

This rabbit polyclonal primary antibody from Antibodies.com is affinity-purified and supplied as an unconjugated IgG reagent raised against histone H4, the highly conserved ~11 kDa core histone that pairs with H2A, H2B, and H3 to form the nucleosome. Endogenous H4 is detected in human, mouse, and rat samples, supporting chromatin biology and epigenetics workflows where total H4 routinely serves as a loading control alongside modification-specific reagents targeting acetylated or methylated H4.

Validated for Western blot and immunofluorescence, the antibody is provided as a 100 µl liquid aliquot in a single-use vial sized for typical experiment panels. For immunofluorescence on nuclear targets, methanol fixation is often well tolerated, and counterstaining with DAPI helps localize the chromatin-associated signal. The unconjugated format allows pairing with user-selected fluorescent or HRP-conjugated secondaries for multiplex detection, including standard anti-rabbit IgG reagents across immunoblotting and immunostaining.

Aliquot the reagent on receipt and store at -20 °C in working volumes to limit freeze-thaw cycles and preserve antibody activity. Handle with standard cold-chain practice and protect from light when the antibody is paired with fluorescent secondaries during downstream detection.