Anti-Histone H4 (Acetyl-K5) Antibody

Recombinant rabbit monoclonal antibody raised against histone H4 acetylated at lysine 5 (H4K5ac), an epigenetic modification associated with transcriptionally active chromatin. Produced by Antibodies.com as an unconjugated primary reagent, it supports Western blot, immunocytochemistry/immunofluorescence (ICC/IF), and immunohistochemistry (IHC) workflows across human, mouse, and rat samples.

The antibody is supplied as 100 µl of recombinant rabbit monoclonal in TBS (pH 7.4) supplemented with 1% BSA, 40% glycerol, and 0.05% sodium azide as preservative. The recombinant expression strategy supports batch-to-batch consistency, while the BSA and glycerol formulation helps stabilize the reagent during routine cold-storage handling. Aliquoting upon first use is recommended to limit repeated freeze-thaw cycles.

H4K5ac sits within the N-terminal tail of histone H4 and is investigated in studies of chromatin remodeling, gene expression regulation, and cellular differentiation programs. Researchers apply this reagent to probe acetylation patterns in nuclear extracts by Western blot, to visualize modified chromatin in fixed cells by ICC/IF, and to localize the mark in tissue sections by IHC.