Anti-Histone H3 (Tri-Methyl K36) Antibody

Tri-methylation of histone H3 at lysine 36 (H3K36me3) is an epigenetic mark deposited along actively transcribed gene bodies and associated with transcription elongation, mRNA processing fidelity, and chromatin state maintenance. Antibodies.com supplies a rabbit polyclonal reagent raised against this specific modification, formulated unconjugated at 1 mg/ml in PBS containing 0.1% sodium azide and 50% glycerol.

The glycerol-supplemented buffer keeps the protein in solution at sub-zero temperatures and protects binding activity through repeated freeze-thaw cycles, supporting long-term storage at -20°C. Each vial contains 100 µl of concentrated stock, enough for dozens of western blot, immunoprecipitation, or chromatin immunoprecipitation experiments at standard working dilutions.

Validated applications

• Western blot detection of H3K36me3 from acid-extracted histone preparations
• Immunohistochemistry on formalin-fixed paraffin-embedded tissue sections
• Immunofluorescence imaging of nuclear staining patterns
• Immunoprecipitation of modified nucleosomes
• Chromatin immunoprecipitation (ChIP) for locus-level occupancy studies
• ChIP-seq workflows that map H3K36me3 distribution genome-wide

The cross-reactivity profile covers histone H3 from human, mouse, and rat samples, making the reagent directly applicable to the three most common mammalian model systems. Investigators studying SETD2-mediated deposition, recruitment of PWWP-domain readers, or loss of H3K36 methylation in cancer and developmental models will find this polyclonal antibody a flexible foundation for both routine detection and quantitative chromatin research.