Anti-Histone H3 (Tri-Methyl K27) Antibody

The trimethylation of histone H3 at lysine 27 (H3K27me3) is a repressive epigenetic mark deposited by EZH2 within Polycomb Repressive Complex 2. This unconjugated rabbit polyclonal reagent from Antibodies.com recognizes that specific post-translational modification on Histone H3 and is supplied at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, packaged as a 50 µl aliquot.

Validated applications include Western blot, immunohistochemistry, immunofluorescence, immunoprecipitation, ChIP, and ChIP-seq workflows. The antibody cross-reacts with Human, Mouse, and Rat samples, which supports comparative epigenomics across mammalian model organisms. Researchers use this reagent to map facultative heterochromatin, trace silencing dynamics during embryonic stem cell differentiation, and profile PRC2 targets in lymphoma, prostate cancer, and other EZH2-relevant disease models. In ChIP-seq, it produces sharp peaks over Polycomb response elements and silenced developmental loci, complementing active marks such as H3K4me3.

Storage is at -20°C in the glycerol-containing buffer; freeze-thaw cycles should be minimized by aliquoting on first use. The polyclonal format provides tolerance for minor sequence variation flanking the K27 epitope while retaining selectivity for the trimethyl state, distinguishing it from H3K27me1, H3K27me2, and H3K27ac marks in multiplexed chromatin studies.