Anti-Histone H3 (Tri-Methyl K27) Antibody

Specific for the tri-methylated Lys27 residue of Histone H3 (H3K27me3), this polyclonal antibody from Antibodies.com targets an epigenetic mark associated with transcriptionally repressed chromatin and Polycomb-group-mediated gene silencing. Raised in rabbit and affinity-purified, the reagent is supplied as an unconjugated preparation at 1 mg/mL in PBS with 0.1% sodium azide and 50% glycerol as a cryoprotectant. Each vial contains 100 µL of ready-to-use liquid stock suitable for chromatin immunoprecipitation workflows, including ChIP-seq, and is also validated for western blot, immunohistochemistry, immunofluorescence, and immunoprecipitation across human, mouse, and rat samples. The 50% glycerol formulation permits storage at -20 °C with minimal freeze-thaw damage, while azide-free aliquoting supports downstream conjugation when needed. Researchers commonly deploy H3K27me3 detection to map facultative heterochromatin domains, follow differentiation-associated silencing events, and characterize PRC2 targets in development and disease models. Each lot is quality-controlled for specificity against the K27me3 mark, and the polyclonal format enables consistent signal across multiple epitopes surrounding the modified residue, which is particularly useful in ChIP-seq for capturing broader genomic regions occupied by Polycomb-repressed chromatin.