Anti-Histone H3 (tri methyl K14) Antibody

A rabbit polyclonal IgG antibody supplied unconjugated and directed against Histone H3 tri-methylated at Lys14. Affinity purified and formulated as a liquid, the reagent is listed by Antibodies.com as a Primary Antibody with applications in Western blot, immunohistochemistry, immunofluorescence, immunoprecipitation, ChIP and ChIP-seq. The manufacturer confirms reactivity with human, mouse and rat samples and validation for WB, IHC, ICC/IF, IP and ChIP.

The immunogen is a synthetic trimethylated peptide corresponding to the region around K14 of human Histone H3 (NP_003520.1), with the reported immunogen sequence GGKAP. The antibody is formulated in phosphate buffered saline at pH 7.3, containing 50% glycerol and 0.01% thiomersal.

The manufacturer lists recommended working dilutions of 1:100–1:500 for Western blot, 1:50–1:200 for IHC, 1:50–1:200 for ICC/IF, 1:50–1:200 for IP, and 1:20–1:100 for both ChIP and ChIP-seq. The target protein has a reported molecular weight of 17 kDa, and the concentration is described as lot specific.