Anti-Histone H3 (T22) Antibody

This polyclonal reagent from Antibodies.com specifically recognizes histone H3 at threonine 22 (H3T22), a nucleosomal post-translational modification linked to chromatin remodeling and transcriptional regulation. Raised in rabbit and supplied as an unconjugated IgG preparation at 1 mg/ml in PBS (approx. pH 7.2) with 0.05% sodium azide preservative, the 50 µl stock is formulated for western blot and immunohistochemistry workflows. The antibody has been validated for reactivity against human, mouse, and rat samples, supporting comparative studies across model organisms and primary cell lines. Polyclonal recognition enables binding to multiple epitopes around the target residue, which can strengthen signal when probing acid-extracted histone preparations or fixed tissue sections. Recommended handling includes brief equilibration to room temperature before use and storage at -20 °C in working aliquots to limit freeze-thaw cycles and preserve antibody activity over extended use.