Anti-Histone H3 (sDi-Methyl R17) Antibody

Produced in rabbit as an unconjugated polyclonal reagent, this Antibodies.com primary antibody recognizes Histone H3 carrying the symmetric dimethyl modification at arginine 17 (H3R17me2s). Arginine methylation on the histone H3 tail is catalysed by protein arginine methyltransferase family members and is studied as a chromatin-associated mark that contributes to transcriptional output and chromatin organization.

The reagent is supplied in a vial as 100 µl at 1 mg/ml in PBS containing 0.1% sodium azide and 50% glycerol as a cryoprotectant; storage at -20 °C in small aliquots is recommended to limit freeze-thaw cycles of the antibody stock.

Validated for western blot, immunohistochemistry, and immunofluorescence workflows, the antibody detects endogenous H3R17 methylation across human, mouse, and rat samples, supporting comparative studies of this mark in cell lines and tissue sections. The unconjugated format allows downstream labelling with fluorophores or HRP, and the polyclonal reagent recognises multiple epitopes around the modified residue, which is helpful when probing modified histone tail peptides in dot blot or peptide array contexts where epitope accessibility can differ from native chromatin.