Anti-Histone H3 (R2) Antibody

Raised in rabbit against the arginine 2 (R2) residue of Histone H3, this polyclonal antibody from Antibodies.com is supplied as 100 µl of a 1 mg/ml stock in phosphate buffered saline (PBS) containing 0.05% sodium azide at approximately pH 7.2. The unconjugated reagent is validated for western blot (WB) and immunohistochemistry (IHC) workflows and has been verified to recognize human, mouse, and rat samples, supporting chromatin and epigenetics research into post-translational modifications at this N-terminal residue, including symmetric and asymmetric arginine methylation states that influence transcription and that exhibit cross-talk with H3K4 methylation.

Each vial is provided as a liquid formulation ready for dilution into standard blocking and washing buffers, and the reagent is intended for research use only across applications such as immunoblotting of acid-extracted histones and IHC staining of tissue sections to map chromatin marks in situ. The polyclonal format allows recognition of multiple epitopes around the R2 region, which can improve detection of modified and unmodified H3 species in nuclear extracts prepared from cultured cells or tissue samples.