Anti-Histone H3 (phospho S10) Antibody

This rabbit polyclonal IgG antibody from Antibodies.com targets Histone H3 phosphorylated at serine 10, a well-established marker of mitotic chromatin condensation. Supplied as an unconjugated reagent in a 200 µl volume, it supports both western blot (WB) and immunohistochemistry (IHC) workflows with human, mouse, and rat samples.

The phospho-S10 epitope sits within the N-terminal tail of Histone H3 and is dynamically added by Aurora B kinase during late G2 and M phase, making this reagent useful for tracking proliferating cells in tissue sections, mapping cell-cycle distribution in lysates, and studying chromatin-modifying pathways in cancer and developmental disease models.

Produced in rabbit and affinity-purified, this polyclonal IgG preparation ships without fluorophore or enzyme conjugation, allowing flexible pairing with HRP-, biotin-, or fluorophore-labeled secondary reagents. Researchers commonly apply it to formalin-fixed paraffin-embedded tissue for IHC and to acid-extracted histone preparations for western blot, where the phospho-S10 band resolves cleanly above baseline signals in mitotically enriched samples.