Anti-Histone H3 (phospho S10) Antibody

Phosphorylation of histone H3 at serine 10 is one of the most widely studied chromatin modifications, and this rabbit polyclonal IgG reagent from Antibodies.com is raised against that exact phospho-epitope.

Validated for Western blot and immunohistochemistry, the unconjugated antibody provides a readout of mitotic chromatin condensation in cultured cells and tissue sections. The reagent is reactive with human, mouse, and rat samples, supporting cross-species studies of cell cycle progression, Aurora kinase and MAPK signaling, and chromatin remodeling during mitosis and meiosis.

Supplied as a 100 µl aliquot, the antibody can be applied directly to acid-extracted histone preparations to detect the characteristic ~17 kDa band on immunoblots, or used on formalin-fixed paraffin-embedded tissue sections to enumerate mitotic figures in tumor and developmental specimens.

Researchers in epigenetics, cancer biology, and stem cell fields apply this phospho-specific reagent to track proliferative status, pair it with total H3 as a loading control, and compare staining intensity across treatments that perturb mitotic entry. The rabbit host and polyclonal format allow recognition of neighbouring residues on the histone tail, useful when adjacent modifications are under investigation.