Anti-Histone H3 (P16) Antibody

This rabbit polyclonal antibody from Antibodies.com targets Histone H3 phosphorylated at Ser16 (H3 pS16), a chromatin mark that rises rapidly during the DNA damage response and apoptotic chromatin condensation. The unconjugated reagent is supplied as 50 µl of a 1 mg/ml stock in phosphate buffered saline (pH ~7.2) with 0.05% sodium azide as a preservative.

Validated for immunohistochemistry on formalin-fixed paraffin-embedded tissue, the antibody cross-reacts with human, mouse, and rat samples, supporting translational studies that span patient biopsies and rodent disease models. As a polyclonal reagent raised in rabbit, it offers broader epitope coverage than monoclonal alternatives and tolerates minor sequence variation between orthologs.

Recommended handling: store refrigerated in the original vial; avoid repeated freeze-thaw cycles by aliquoting on first use; protect from light during extended incubations. Sodium azide is incompatible with some HRP-conjugated secondary workflows, so rinse thoroughly or use azide-free buffer when coupling to enzyme detection.