Anti-Histone H3 (P16) Antibody

Raised in rabbit against a synthetic peptide (designated P16) within the Histone H3 sequence, this unconjugated polyclonal antibody from Antibodies.com is validated for immunohistochemistry (IHC) on human, mouse, and rat tissue. The unconjugated format provides laboratories flexibility to pair the reagent with their preferred fluorophore, biotin, or enzyme-based detection chemistry for chromogenic and fluorescent staining workflows.

The reagent is supplied at approximately 1 mg/ml in phosphate buffered saline (PBS) containing 0.05% sodium azide at pH 7.2, packaged as a 100 µl vial. For storage, keep refrigerated and avoid repeated freeze-thaw cycles to preserve activity across multiple staining experiments.

Histone H3 is a core nucleosomal protein whose post-translational modifications, including methylation, acetylation, and phosphorylation, regulate chromatin architecture and gene expression. Polyclonal antibodies raised against Histone H3 serve as specific markers for epigenetic studies and as reference signals in chromatin immunoprecipitation and nuclear counterstaining, supporting research in gene regulation, cellular differentiation, and chromatin biology.