Anti-Histone H3 (Mono-Methyl R8) Antibody

This rabbit polyclonal antibody from Antibodies.com is raised against histone H3 mono-methylated at arginine 8 (H3R8me1), an epigenetic mark linked to transcriptional regulation at active gene promoters.

The reagent is supplied at 1 mg/ml in PBS containing 0.1% sodium azide and 50% glycerol, an anti-freeze formulation that keeps the antibody stable at -20 °C through repeated freeze-thaw cycles. Each vial contains 100 µl of unconjugated IgG, sufficient for dozens of western blots, immunostaining runs, or chromatin immunoprecipitation assays.

Validated for chromatin biology workflows, the antibody is suited to Western blot, immunohistochemistry, immunofluorescence, immunoprecipitation, and chromatin immunoprecipitation, including ChIP-seq library preparation. The polyclonal host enables recognition of multiple epitopes surrounding the mono-methyl R8 residue, which is useful when neighbouring sequence context varies between loci.

Reactivity has been confirmed in human, mouse, and rat samples, supporting translational studies that bridge cell-line work with primary tissue from rodent disease models and clinical specimens. Because the target is a post-translational modification rather than the underlying H3 protein, researchers commonly pair this reagent with antibodies against unmodified H3 or alternative methylation states to map modification-specific chromatin at promoters, enhancers, and repetitive elements.

For long-term storage, aliquot upon first use and store at -20 °C to minimize freeze-thaw cycles; the 50% glycerol buffer prevents ice crystal damage during routine handling. This product is intended for research use only.