Anti-Histone H3 (Mono-Methyl R2) Antibody

This rabbit polyclonal antibody from Antibodies.com specifically recognizes histone H3 monomethylated at residue R2 (arginine 2), an epigenetic modification associated with chromatin remodeling and transcriptional regulation. Raised in rabbit and purified as an unconjugated polyclonal reagent, the antibody is supplied at 1 mg/ml in a PBS buffer containing 0.1% sodium azide and 50% glycerol, shipped as a 100 µl aliquot ready for dilution into standard assay buffers.

Validated for western blot (WB), immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP), the reagent enables detection of the H3R2me1 mark in samples derived from human, mouse, and rat sources. Researchers studying histone arginine methylation, PRMT family activity, or nuclear signaling cascades commonly deploy this antibody in ChIP-seq or ChIP-qPCR workflows to map monomethyl-R2 enrichment at gene promoters and regulatory elements, while the same lot supports parallel immunofluorescence imaging of nuclear staining patterns and western blot quantification of acid-extracted histone preparations.

For routine use, small working aliquots should be prepared to limit freeze-thaw cycles of the glycerol stock; long-term storage at -20 °C is recommended, with the sodium azide preservative preventing microbial contamination during refrigerated handling of working stocks. The unconjugated format allows direct conjugation to fluorophores, HRP, or biotin depending on the downstream detection chemistry required by the experiment, making this polyclonal reagent a flexible building block across both chromatin immunoprecipitation and imaging-based epigenetic studies.