Anti-Histone H3 (Mono-Methyl K9) Antibody

This rabbit polyclonal antibody from Antibodies.com specifically recognizes Histone H3 mono-methylated at lysine 9 (H3K9me1), a post-translational chromatin mark enriched over active gene bodies and euchromatic regions. Researchers deploy it across multiple experimental platforms, including chromatin immunoprecipitation followed by deep sequencing (ChIP-seq) for genome-wide profiling of monomethylation landscapes, conventional ChIP for targeted locus analysis, immunofluorescence and immunohistochemistry to visualize H3K9me1 distribution in tissue sections and cultured cells, immunoprecipitation to pull down modified nucleosomes, and Western blot detection of the methylated histone band.

Cross-reactivity has been validated against human, mouse, and rat samples, making the reagent broadly applicable across standard mammalian model systems. The antibody is supplied as 100 µl of unconjugated antiserum at 1 mg/ml in PBS supplemented with 0.1% sodium azide and 50% glycerol as a cryoprotectant, allowing convenient handling and storage at -20 °C without loss of activity. For long-term use, aliquot upon receipt and minimize freeze-thaw cycles to preserve binding performance across repeat experiments in epigenetics, developmental biology, and oncology workflows.