Anti-Histone H3 (Mono-Methyl K79) Antibody

Rabbit polyclonal reagent from Antibodies.com raised against histone H3 mono-methylated at lysine 79 (H3K79me1), an epigenetic mark deposited by the DOT1L methyltransferase and associated with active transcription, transcriptional elongation, and chromatin remodeling across active gene bodies. The antibody is supplied as 100 µl of unconjugated protein at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, and is validated for western blot, immunohistochemistry, immunofluorescence, immunoprecipitation, chromatin immunoprecipitation (ChIP), and ChIP-seq workflows. Reactivity has been confirmed in human, mouse, and rat samples, supporting comparative chromatin studies in cell lines, primary tissues, and rodent disease models.

Research applications include profiling H3K79me1 occupancy at promoters and coding regions, tracking methylation state changes during differentiation or oncogenic transformation, and pairing the antibody with DOT1L inhibitor treatments to dissect methyltransferase-dependent gene regulation. The polyclonal format provides robust recognition of the mono-methyl mark on nucleosomal substrates. Store the vial at 4 °C for short-term use; for longer term storage, aliquot and freeze at -20 °C, avoiding repeated freeze-thaw cycles to preserve antibody titer and binding performance.