Anti-Histone H3 (Mono-Methyl K4) Antibody

Raised in rabbit and affinity-purified to greater than 95% purity by SDS-PAGE, this polyclonal reagent from Antibodies.com specifically recognises histone H3 only when mono-methylated at lysine 4 (H3K4me1), without cross-reacting with the di- or tri-methylated forms. The immunogen is a synthetic KLH-conjugated peptide corresponding to residues 1-100 of human histone H3 bearing the mono-methyl K4 modification, and the resulting antibody detects the endogenous target at an apparent molecular weight of approximately 17 kDa across human, mouse and rat samples. Supplied at 1 mg/ml in PBS containing 0.1% sodium azide and 50% glycerol, the unconjugated antiserum is offered in a 50 µl volume and is validated for a broad range of chromatin and epigenetics workflows, including Western blot, immunohistochemistry, immunofluorescence, immunoprecipitation, ChIP and ChIP-seq. Researchers studying enhancer-associated histone marks, transcription regulation and chromatin state will appreciate the antibody's sensitivity for endogenous H3K4me1, making this Antibodies.com product a reliable tool for profiling mono-methylation at lysine 4 of histone H3 in a variety of model systems.