Anti-Histone H3 (Mono-Methyl K27) Antibody

This rabbit polyclonal reagent from Antibodies.com recognizes histone H3 mono-methylated at lysine 27 (H3K27me1), a chromatin modification enriched at active and poised enhancers and commonly profiled alongside H3K27me3 to monitor PRC2-regulated loci. The unconjugated antibody is supplied as 100 µl at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, and it has been validated for Western blot, immunohistochemistry, immunofluorescence, immunoprecipitation, chromatin immunoprecipitation and ChIP-seq across human, mouse and rat samples. The polyclonal format provides tolerance for single-residue methylation marks while remaining compatible with peptide-blocking controls. Researchers apply this reagent to track enhancer activity dynamics, map Polycomb-repressive complex occupancy changes during differentiation, and profile H3K27 methylation transitions in development and disease models.