Anti-Histone H3 (mono methyl K14) Antibody

Affinity-purified rabbit polyclonal IgG antibody raised against a synthetic monomethylated peptide corresponding to the K14 region of human Histone H3 (sequence PRKQL). Supplied unconjugated by Antibodies.com, the reagent is validated for Western blot, immunohistochemistry, and immunocytochemistry/immunofluorescence applications and has been shown to react with human, mouse, and rat samples.

The target is the mono-methylated Lys14 form of Histone H3, a protein with a molecular weight of 17 kDa. The antibody is provided as a liquid in phosphate buffered saline (pH 7.3) containing 50% glycerol and 0.01% thiomersal, with lot-specific concentration. Recommended working dilutions are 1:500–1:1,000 for Western blot, 1:50–1:200 for IHC, and 1:20–1:50 for ICC/IF.

The product is shipped at 4°C and should be aliquoted and stored at -20°C upon receipt, with freeze/thaw cycles avoided.