Anti-Histone H3 (L20) Antibody

An affinity reagent for nuclear protein analysis, this polyclonal antibody from Antibodies.com is raised in rabbit against the L20 epitope of Histone H3 and is supplied unconjugated for direct use in antibody-based detection assays. The product is provided as 50 µl of liquid at 1 mg/ml in phosphate buffered saline (PBS) containing 0.05% sodium azide at approximately pH 7.2.

Validated applications include western blot (WB) and immunohistochemistry (IHC). The antibody recognizes Histone H3 in human, mouse, and rat samples, enabling cross-species comparison in cell lysates, frozen or paraffin-embedded tissue sections, and chromatin-enriched fractions. The polyclonal format offers multi-epitope recognition of the H3 tail, supporting studies of global histone levels, post-translational modification patterns, and normalization of nuclear protein loading across blots and stained tissue sections.

For best results, store refrigerated, protect from light, and minimize freeze-thaw cycles to preserve antibody activity across multiple experiments.