Anti-Histone H3 (Di-Methyl K4) Antibody

Raised in rabbit and affinity-purified, this polyclonal reagent from Antibodies.com is designed to detect Histone H3 when di-methylated at lysine 4 (H3K4me2), an epigenetic mark associated with actively transcribed chromatin. The antibody is supplied as a 50 µl liquid at a lot-specific concentration, formulated in Phosphate Buffered Saline (pH 7.3) with 50% glycerol and 0.01% Thiomersal, and it is unconjugated for maximum flexibility in downstream labelling. The immunogen is a synthetic dimethylated peptide corresponding to the RTKQT motif surrounding K4 of human histone H3 (NP_003520.1), and the target migrates at approximately 17 kDa. Validated applications include Western blot (1:500–1:1,000), IHC (1:50–1:200), ICC/IF (1:50–1:200), immunoprecipitation (1:50–1:200), ChIP (1:50–1:200), and ChIP-seq (1:50–1:200), making the reagent a strong fit for chromatin, transcription, and epigenetics workflows. The host species is rabbit, the clonality is polyclonal, and the isotype is IgG. Reactivity has been confirmed in human, mouse, and rat samples, with a research-use-only disclaimer covering diagnostic or therapeutic applications. For handling, the vial is shipped at 4°C and should be aliquoted and stored at –20°C on arrival, with repeated freeze/thaw cycles avoided to preserve activity. Suitable secondary antibodies, including AP, biotin, FITC, and HRP conjugates of goat anti-rabbit IgG, are listed on the product page to support the chosen detection method.