Anti-Histone H3 (Di-Methyl K27) Antibody

This rabbit polyclonal antibody recognizes histone H3 when di-methylated at lysine 27 (H3K27me2), an epigenetic mark associated with transcriptional repression and facultative heterochromatin. Provided by Antibodies.com as a 50 µL vial at 1 mg/mL in PBS with 0.1% sodium azide and 50% glycerol, the reagent is unconjugated and validated across multiple assay platforms including Western blot, immunohistochemistry, immunofluorescence, immunoprecipitation, chromatin immunoprecipitation (ChIP), and ChIP-seq in human, mouse, and rat samples.

The di-methyl K27 mark on histone H3 is deposited by Polycomb repressive complex 2 (PRC2) and is commonly interrogated in studies of X-chromosome inactivation, stem cell differentiation, embryonic development, and cancer epigenetics. Polyclonal reactivity against H3K27me2 provides broader epitope coverage than many monoclonal alternatives, supporting consistent detection of the modification across varied sample preparations and fixation conditions.

Storage at -20°C is recommended; the glycerol-containing buffer protects against freeze-thaw damage, and aliquoting upon first use preserves antibody activity across repeated experiments. Researchers evaluating repressive chromatin states, PRC2-mediated silencing, or histone modification dynamics will find this reagent a practical tool for both targeted protein detection and genome-wide mapping of H3K27me2 distribution.