Anti-Histone H3 (di methyl K27) Antibody

Primary antibody from Antibodies.com: a rabbit polyclonal raised against Histone H3 di-methylated at Lys27. It is supplied as an unconjugated liquid preparation of the IgG isotype, produced in rabbit and affinity purified. It reacts with human, mouse and rat samples. Applications include Western blotting (WB), immunohistochemistry (IHC), immunofluorescence (IF/ICC), immunoprecipitation (IP), chromatin immunoprecipitation (ChIP) and ChIP-seq. According to the manufacturer's specification, the immunogen is a synthetic dimethylated peptide around K27 of human histone H3 (NP_003520.1), and the corresponding peptide sequence is ARKSA. The target protein has a molecular weight of 17 kDa. The formulation is Phosphate Buffered Saline pH 7.3, with 50% glycerol and 0.02% sodium azide, and the concentration is lot specific. Recommended dilutions are 1:500-1:1,000 for WB, 1:50-1:200 for ICC/IF, 1:50-1:200 for IP, 1:20-1:100 for ChIP and 1:20-1:100 for ChIP-seq. The product is shipped at 4°C; on receipt it should be aliquoted and stored at -20°C, avoiding repeated freeze/thaw cycles.